Antibacterial Potential of Kepok Sobo Londo Banana Peel (Musa paradisiaca L.) Against Diarrhea Causing Bacteria Enterohemorrhagic Escherichia coli

Abstract

The administration of excessive doses of antibiotics and frequency of diarrhea outbreaks caused by Enterohemorrhagic E. coli (EHEC) need to be reexamined, and one of it measures is considering the potency of local plants, such as kepok sobo londo banana. This study is conducted to examine the antibacterial potential of kepok sobo londo banana peel extract (Musa paradisiaca L.) through its inhibitory activity on the growth of EHEC. The bacterial isolates were molecularly identified using specific primers encoding EHEC virulence genes, namely stx1 and eae, respectively. Banana peel samples were extracted by maceration method and tested for phytochemicals using GC-MS. Antibacterial testing was carried out using the agar well diffusion method, as well as MIC and MBC assay. The data from GC-MS analysis results in the identification of main antibacterial compounds contained in the extract, namely fatty acids, tannin alkaloids, and phenols, respectively. The results of the antibacterial assay using agar well diffusion method for inhibition of EHEC bacteria by kepok sobo londo banana peel extract were found to be greatest at a concentration of 28% which is considered as intermediate inhibitory power. Obtained MIC dan MBC value of extract occurred at concentration of 17% and 22%, respectively against EHEC.